Research

At the core of EvoCell’s technological development is the application of evolutionary theory. Over the last number of years we have uncovered how the mechanisms that govern pluripotency in mammals evolved from lower animals. Embryological material from lower animals is vastly more accessible than from mammals, and we have exploited this in the preparation of cell extracts.
Because the gene regulatory networks that govern pluripotency have been conserved during evolution, the proteins in these extracts are compatible with the DNA sequences that govern expression of pluripotency genes in mammalian cells. We have identified molecules in oocytes from basal vertebrates encoding mammalian gene orthologues. These transcription factors are well known for their capacity to maintain pluripotency and participate in the reprogramming of somatic cells. The long term goal of our research platform is to identify new molecules and produce a cocktail of reprogramming factors that can be delivered to somatic cells. The technology has been patented and proof of principle with one molecule has been demonstrated.
Target molecules include epigenetic remodelers unique to eggs as well as pluripotency factors. Our strategy for identifying such factors is based on using next generation sequencing technology to identify oocyte molecules among basal vertebrates with conserved developmental programs.

Recent Publications

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